bioWORLD'S Urease Activity Detection Kit utilizes the Berthelot Reaction to detect urease activity (by levels of ammonia production) colorimetrically. Urease catalyzes the hydrolysis of urea to produce ammonia and carbamate; the carbamate produced is subsequently degraded by spontaneous hydrolysis to produce another ammonia and carbonic acid.
Urea + H2O + 2H --> Urease --> 2NH3 + CO2
The ammonium ions formed react with two reagents to give a green dye (2.2 dicarboxylindophenol) which can be measured at 670 nm. The kit components are sufficient for 480 assays using a microplate.
Catalog No. | : | 10140002-1 | Size | : | 500 Assay(s) |
BioWorld 为最具创新性的药物开发提供动态的情报信息,推动企业的发展,为疾病的治疗提供帮助。BioWorld 全球记者和编辑实地报道突破性新闻并提供重要的观点,报道包括全球数百个药物的研发进展、支持这些药物开发的公司、推动市场发展的商业交易、和有挑战及指导性作用的政策法规。