Sputum DNA Collection, Preservation and Isolation Kit is an all-in-one method designed for:

• Simple and non-invasive sputum collection; • Preservation of DNA in sputum samples at ambient temperature; • Isolation of high quality DNA.

The Sputum DNA Collection, Preservation and Isolation Kit contains 50 individual Sputum Collection and Preservation Devices, as well as the required reagents for the subsequent isolation of sputum DNA from the preserved samples. Each of the 50 individual kits consists of three components: (1) Sputum Collection Tube; (2) Collection Funnel and; (3) Norgen's Sputum DNA Preservative contained within sealed ampoules. Sputum samples (either induced or non-induced) are collected by the patient by first rinsing the mouth thoroughly with water. Following this step the patient must then cough deeply to loosen sputum and then expectorate/spit inside the Collection Funnel which has been assembled with the Collection Tube. Following collection of the sputum sample, the Collection Funnel is removed and the contents of the Preservative ampoule are then added and mixed with the collected sputum and the Collection tube is labeled. The Sputum Collection Tube is subsequently sent to the laboratory for DNA isolation and analyses. The sputum DNA in preserved samples is stable for more than two years at ambient temperature. The kit is ideal for collecting, preserving and isolating DNA samples for numerous research based applications, as well as epidemiological and population studies.

SPUTUM DNA PRESERVATIVE Norgen's Sputum DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh sputum samples. The preservative prevents the growth of Gram-positive bacteria, Gram-negative bacteria and fungi and inactivates viruses allowing the samples to be handled and shipped in a safer manner. In addition, the samples are preserved allowing their storage and shipment at ambient temperature, eliminating the requirement for refrigeration or freezing, decreasing costs. The components of the preservative will allow the collected samples to be stored for more than 2 years without any detectable DNA degradation.

NUCLEIC ACID ISOLATION FROM PRESERVED SPUTUM Sputum DNA is isolated from the preserved samples either according to ethanol precipitation or can be performed using Sputum DNA Isolation Kit (Cat#10760131-1). The preservative is also compatible with other DNA isolation methods.

FEATURES AND BENEFITS

• Non-invasive sample collection - Higher donor compliance and lower costs due to non-invasive sample collection • Convenient, all-in-one kit - Sputum sample collection, preservation and DNA isolation all with one convenient, affordable kit • Safer handling - The provided Sputum DNA Preservative is designed for rapid cellular lysis, prevents the growth of Gram-negative and Gram-positive bacteria and fungi and also inactivates viruses allowing the samples to be handled and shipped in a safer manner. • DNA is stable for years at ambient temperature - Sputum DNA preserved with Norgen's Sputum DNA Preservative is stable at room temperature for over 2 years, thereby reducing storage costs associated with fridges and freezers. • Compatible with most DNA isolation methods - DNA can be isoated from the samples via ethanol precipitation using the provided reagents. Alternatively, DNA can be isolated using other methods, such as Sputum DNA Isolation Kit (Cat# 10760131-1) which uses spin columns. • High quality DNA is suitable for sensitive downstream assays - Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR Shipping Accessories can be purchased separately - Norgen also provides shipping accessories (envelopes and bags) which meet all standards and regulations for safe shipping of biological samples.

KIT CONTENTS

1. Individual Sputum DNA Collection and Preservation Device: a. Collection Tubeb. Collection Funnelc. Preservative ampouled. Absorbant pade. Post-collection labelf. Patient instructions 2. Sputum Liquefier 3. Proteinase K 4. Purification Additive 5. Product Insert

SHELF LIFE AND HANDLINGThis kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at 4°C (stable for 2 years) or at -20oC for long term storage. All other kit components should be kept tightly sealed and stored at room temperature (15 - 25 °C) for up to 3 years without any reduction in kit performance. The Collection Tube, Collection Funnel and the Device container of each Individual Sputum DNA Collection and Preservation Device are recyclable.

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