Applications:
OnePass™ GST-Tagged Recombinant Protein Purification column consists of Glutathione-Separopore®4B-CL conveniently prepacked column cartridges for low pressure flow chromatography. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated highly crosslinked 4% agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity and could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel. Reduced glutathione is covalently linked to Separopore® 4B-CL for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function. The OnePass™ prepacked Glutathione- Separopore®spin columns simply purification of GST-tagged proteins.
Technical specifications:
References:
Related products
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Catalog No. | : | 20170004-1 | Size | : | 15 mL |
BioWorld 为最具创新性的药物开发提供动态的情报信息,推动企业的发展,为疾病的治疗提供帮助。BioWorld 全球记者和编辑实地报道突破性新闻并提供重要的观点,报道包括全球数百个药物的研发进展、支持这些药物开发的公司、推动市场发展的商业交易、和有挑战及指导性作用的政策法规。